%PDF-1.4 % 17 0 obj <>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>>>/BBox[0 0 584.96 782.99]/Length 133>>stream x???Dާ :D@N?$ D?َ?)hżWnq`_Wt>U>q{t>stream Biochemical and Structural Study of RuvC and YqgF from Deinococcus radiodurans 10.1128/mbio.01834-22 Yiyang Sun Jieyu Yang Guangzhi Xu Kaiying Cheng Research Article Research Article Holliday junction (HJ) RuvC RNase homologous recombination (HR) Deinococcus protein structure American Society for Microbiology Sun et al. Copyright © 2022 Sun et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. 20220824 2022 ABSTRACT Deinococcus radiodurans possesses robust DNA damage response and repair abilities, and this is mainly due to its efficient homologous recombination repair system, which incorporates an uncharacterized Holliday junction (HJ) resolution process. D. radiodurans encodes two putative HJ resolvase (HJR) homologs: RuvC (DrRuvC) and YqgF (DrYqgF). Here, both DrRuvC and DrYqgF were identified as essential proteins for the survival of D. radiodurans. The crystal structures and the biochemical properties of DrRuvC and DrYqgF were also studied. DrRuvC crystallized as a homodimer, while DrYqgF crystallized as a monomer. DrRuvC could preferentially cleave HJ at the consensus 5?(G/C)TC?G/C)-3?sequence and could prefer using Mn2+ for catalysis in vitro, which would be different from the preferences of the other previously characterized RuvCs. On the other hand, DrYqgF was identified as a Mn2+-dependent RNA 5′??exo/endonuclease with a sequence preference for poly(A) and without any HJR activity. IMPORTANCE Deinococcus radiodurans is one of the most radioresistant bacteria in the world due to its robust DNA damage response and repair abilities, which are contributed by its efficient homologous recombination repair system. However, the late steps of homologous recombination, especially the Holliday junction (HJ) resolution process, have not yet been well-studied in D. radiodurans. We characterized the structural and biochemical features of the two putative HJ resolvases, DrRuvC and DrYqgF, in D. radiodurans. It was identified that DrRuvC and DrYqgF exhibit HJ resolvase (HJR) activity and RNA exo/endonuclease activity, respectively. Furthermore, both DrRuvC and DrYqgF digest substrates in a sequence-specific manner with a preferred sequence that is different from those of the other characterized RuvCs or YqgFs. Our findings provide new insights into the HJ resolution process and reveal a novel RNase involved in RNA metabolism in D. radiodurans. 20220628 20220725 2150-7511 Adobe LiveCycle PDF Generator; modified using iText® 5.5.13.2 ©2000-2020 iText Group NV (AGPL-version)2022-08-26T17:57:21-07:00 endstream endobj 22 0 obj <>stream x??þۺһ